手动移液器（可调式 / 固定式微量液体处理系统）刑事技术实验室与司法鉴定合规应用介绍
作者：毕思特科技 来源：毕思特科技 浏览数：16 发布时间：2026/4/13 8:34:17
手动移液器（可调式 / 固定式微量液体处理系统）刑事技术实验室与司法鉴定合规应用介绍
【重要合规前置声明】本产品为刑事技术实验室、法医司法鉴定专用计量级微量液体处理器具，仅面向具备法定法医物证鉴定、刑事技术检验、毒物毒品分析资质的公安、检察、司法行政、司法鉴定等国家机关及授权鉴定机构定向研发、生产与销售，不向任何个人、无资质企业及非授权单位提供。本产品的使用、校准与维护，必须严格遵守《中华人民共和国刑事诉讼法》《中华人民共和国计量法》《中华人民共和国司法鉴定程序通则》《法庭科学 DNA 实验室规范》《实验室生物安全通用要求》及 EN/ISO 8655 国际计量标准，必须由经过专业培训的实验室技术人员规范操作，定期开展计量校准与性能验证，严禁无资质人员违规使用，严禁因操作不当、未按期校准导致涉案检材交叉污染、检验数据失真、司法鉴定证据失效。
产品概述
手动移液器（可调式 / 固定式微量液体处理系统），是刑事科学技术实验室中实现微量液体精确转移、分液与混合的核心手动计量器具，也是法医 DNA 检验、毒物毒品分析、血清学鉴定、微量物证理化检验全流程中不可或缺的基础性关键设备。本产品专为法医实验室严苛的检材处理需求设计，通过 0.1μl 至 10ml 的宽范围可调取液量程，搭配符合人体工学的轻量化操作结构，可在 PCR 反应体系配制、DNA 提取纯化、标准溶液制备、酶试剂添加等关键实验步骤中，实现微量液体体积的高精度、高重复性转移操作，从实验源头保障后续检测数据的准确性、科学性与可追溯性，是维护刑事技术实验室检测质量体系、保障涉案物证检验结果司法证据效力的核心基础器具。
技术原理
常规可调式手动移液器核心基于空气置换原理工作，针对高粘度、高蒸气压、易起泡的特殊液体（如组织裂解液、有机提取试剂、高粘度蛋白溶液），可搭配专用吸头采用正置换原理实现精准移液。空气置换模式下，操作人员按压活塞通过弹簧与精密机械装置驱动，在吸头内部形成稳定的负压环境，将定量液体精准吸入吸头内；通过控制活塞的正向行程，在吸头内形成正压，将液体完全排出，实现微量液体的定量转移。
产品的移液精度核心来源于精密加工的活塞 - 气缸密封系统、标准化校准机构，以及与之匹配的高质量无酶无热源一次性吸头，三者共同构成密闭的计量体系，确保移液体积的精准可控。移液体积的设定通过数字旋钮与可视化视窗调节内部活塞的机械行程实现，可快速完成量程的精准调整，同时通过独立的管嘴连件确保吸头与移液器之间的绝对密封性，杜绝漏液、体积偏差等问题，完全符合法医微量检材检验对移液精度的极致要求。
核心功能与合规实战价值
产品提供 0.1μl 超微量至 10ml 的全量程覆盖，可全面满足刑事技术实验室从超微量 PCR 体系构建到大宗样本前处理的全流程移液需求，每支移液器出厂前均严格按照 EN/ISO 8655 国际计量标准进行全量程校准，移液的不准确度与不精确度均优于国际标准要求，确保每一次移液操作的体积精准可控，检验数据具备完整的计量溯源性，完全符合司法鉴定机构资质认定、实验室认可对计量器具的核心管理要求。
产品采用轻量化人体工学设计，握持手感舒适，活塞按压力度适中，可有效降低实验室人员长时间连续操作带来的手部疲劳，提升批量样本处理的工作效率。移液器下半支（与样本、试剂接触部分）采用可拆卸式设计，可耐受 121℃、20 分钟的高温高压灭菌处理，便于使用前后的彻底净化消毒，从硬件层面杜绝不同涉案检材、不同批次实验之间的交叉污染，尤其适配法医微量生物检材、珍贵涉案物证的处理需求，最大限度保障检材的原始性与检验结果的真实性。
产品配套完善的校准与维护体系，提供免费在线校准技术支持与标准化校准流程指导，实验室人员可借助标准砝码与纯化水，便捷完成设备的定期性能验证与量程校准，确保移液器在全生命周期内持续满足法定计量要求，保障长期使用的精度稳定性，避免因计量器具失准导致的检验结果无效。同时提供 8 道、12 道多道移液器规格，专为 96 孔板批量操作优化设计，配备同步管嘴推出器，可大幅提升批量 DNA 提取、PCR 体系配制、法医 DNA 数据库建设样本处理的工作效率；液头支持 360 度旋转，可适配生物安全柜、超净台内狭小空间的操作需求，每道通道配备独立活塞，日常维护与故障排查简便快捷。
合规应用场景
本产品仅可在具备法定检验资质的刑事技术实验室、司法鉴定机构内，由专业培训人员规范操作，核心合规应用场景包括四大类：一是法医 DNA 提取与 PCR 反应体系构建，在磁珠法、柱提法等 DNA 提取流程中，精准移取裂解液、结合液、洗涤液与洗脱液，在 STR 分型、PCR 扩增反应体系配制中，准确添加模板 DNA、引物、探针、dNTPs 与 DNA 聚合酶，是获得有效 DNA 分型图谱、完成个体识别的核心基础；二是毒物毒品与理化定量分析，用于配制不同浓度的毒（药）物标准溶液、内标溶液，精准移取涉案血液、尿液等生物检材的上清液，保障气相色谱、液相色谱 - 质谱等仪器定性定量分析结果的准确性；三是血清学与免疫学检验，在酶联免疫吸附试验、血型鉴定、抗体抗原反应等实验中，精确分配待测样品、抗体、酶底物等试剂，确保反应条件的标准化与结果的可重复性；四是微量物证处理与实验室标准体系建设，用于微量痕迹检材的提取溶剂精准转移，以及实验室标准曲线溶液、缓冲体系、质控样品的配制，支撑刑事技术实验室标准化质量体系的稳定运行。
核心技术参数
产品量程范围覆盖 0.1μl 至 10000μl（10ml），全面适配实验室超微量、微量、常量液体处理需求，出厂校准与定期校准严格遵循 EN/ISO 8655 国际计量标准，移液精度优于标准规定要求。通道配置包含单道可调式移液器，以及适配 96 孔板操作的 8 道、12 道多道移液器，配备高清数字视窗，量程设定清晰直观，下半支可耐受 121℃高温高压消毒，整体采用符合人体工效学的轻量化设计。多道移液器配备同步管嘴推出器，液头支持 360 度旋转，每道通道配备独立活塞，便于日常维护与性能校准。
合规实战应用案例
- 某市公安局刑事技术支队受理一起系列入室盗窃案件，在数十起案发现场仅提取到门窗把手、饮料瓶上的微量接触性脱落细胞检材，检材中 DNA 含量极低，属于疑难微量生物检材，对 PCR 反应体系配制的移液精度要求极高。实验室检验人员使用本系列单道可调式微量移液器，精准完成 1μl 级别模板 DNA、引物、聚合酶等试剂的移取与体系配制，全程通过可高温灭菌的下半支设计杜绝交叉污染，移液精度完全符合 ISO 计量标准，最终成功从 12 份微量检材中获得完整的嫌疑人 STR 分型图谱，经全国法医 DNA 数据库比对成功锁定前科人员张某某，破获该系列跨区域盗窃案件 40 余起，本产品的高精度移液能力、防交叉污染设计，为疑难微量检材的成功检验提供了关键基础保障。
- 某市发生一起重大投毒案件，受害人因食用被投毒的食物后中毒身亡，法医提取了受害人胃内容物、血液、剩余食物等涉案检材，需要通过液相色谱 - 质谱联用技术完成毒物的定性定量分析。检验过程中，需要配制梯度浓度的毒物标准溶液、内标溶液，对移液的准确性要求极高，直接影响定量结果的司法有效性。检验人员使用本系列移液器，精准完成不同浓度标准品的梯度稀释与移取，严格按照校准流程完成移液前的性能验证，确保标准曲线配制的准确性，最终成功在检材中检出剧毒农药成分，并完成精准的定量分析，为案件定性、司法诉讼提供了科学、准确、可追溯的定量检验结果，助力公安机关快速抓获投毒嫌疑人，完成案件的全链条侦办。
Application Introduction of Manual Pipette (Adjustable/Fixed Micro Liquid Handling System)
【Important Compliance Pre-Declaration】This product is a metrological grade micro liquid handling appliance dedicated to criminal technology laboratories and forensic judicial authentication. It is only developed, produced and sold for state organs and authorized authentication institutions with legal qualifications of forensic material evidence identification, criminal technical inspection, and toxic and drug analysis, including public security organs, procuratorates, judicial administrative organs, and judicial authentication institutions, and will not be provided to any individual, unqualified enterprise or non-authorized unit. The use, calibration and maintenance of this product must strictly abide by national laws, regulations and international standards such as the Criminal Procedure Law of the People's Republic of China, Metrology Law of the People's Republic of China, General Rules for Judicial Authentication Procedures of the People's Republic of China, Forensic Science DNA Laboratory Specification, General Requirements for Laboratory Biosafety and EN/ISO 8655. It must be operated in a standardized manner by laboratory technicians who have received professional training, and metrological calibration and performance verification must be carried out regularly. It is strictly prohibited for unqualified personnel to use it in violation of regulations, and it is strictly prohibited to cause cross-contamination of involved evidence, distortion of test data and invalidation of judicial authentication evidence due to improper operation and failure to calibrate on schedule.
Product Overview
The Manual Pipette (Adjustable/Fixed Micro Liquid Handling System) is the core manual metering instrument for accurate transfer, dispensing and mixing of micro liquids in criminal science and technology laboratories, and also an indispensable basic key equipment in the whole process of forensic DNA inspection, toxic and drug analysis, serological identification, and physical and chemical inspection of trace evidence. This product is specially designed for the stringent sample processing needs of forensic laboratories. With a wide adjustable pipetting range from 0.1μl to 10ml, matched with a lightweight operation structure in line with ergonomics, it can realize high-precision and high-repeatability transfer operation of micro liquid volume in key experimental steps such as PCR reaction system preparation, DNA extraction and purification, standard solution preparation, and enzyme reagent addition. It ensures the accuracy, scientificity and traceability of subsequent test data from the source of the experiment, and is the core basic instrument for maintaining the testing quality system of criminal technology laboratories and ensuring the judicial evidence validity of the inspection results of involved evidence.
Technical Principle
The conventional adjustable manual pipette works based on the air displacement principle. For special liquids with high viscosity, high vapor pressure and easy foaming (such as tissue lysate, organic extraction reagent, high viscosity protein solution), it can be matched with special pipette tips to achieve accurate pipetting by positive displacement principle. In the air displacement mode, the operator presses the piston to drive through the spring and precision mechanical device, forming a stable negative pressure environment inside the pipette tip, and sucking the quantitative liquid into the pipette tip accurately. By controlling the forward stroke of the piston, a positive pressure is formed in the pipette tip to completely discharge the liquid and realize the quantitative transfer of micro liquid.
The pipetting accuracy of the product comes from the precision machined piston-cylinder sealing system, standardized calibration mechanism, and matched high-quality enzyme-free and pyrogen-free disposable pipette tips. The three together form a closed metering system to ensure the precise control of pipetting volume. The setting of pipetting volume is realized by adjusting the mechanical stroke of the internal piston through the digital knob and visual window, which can quickly complete the accurate adjustment of the range. At the same time, the absolute tightness between the pipette tip and the pipette is ensured through the independent nozzle connector, eliminating the problems of liquid leakage and volume deviation, which fully meets the extreme requirements for pipetting accuracy in forensic trace sample inspection.
Core Functions and Compliance Value
The product provides a full range coverage from 0.1μl ultra-micro to 10ml, which can fully meet the whole process pipetting needs of criminal technology laboratories from ultra-micro PCR system construction to bulk sample pre-processing. Each pipette is strictly calibrated in full range according to the EN/ISO 8655 international metrology standard before delivery. The inaccuracy and imprecision of pipetting are better than the requirements of international standards, ensuring that the volume of each pipetting operation is accurate and controllable, and the test data has complete metrological traceability, which fully meets the core management requirements for metering instruments in the qualification certification and laboratory accreditation of judicial authentication institutions.
The product adopts a lightweight ergonomic design with comfortable holding hand feeling and moderate piston pressing force, which can effectively reduce hand fatigue caused by long-time continuous operation of laboratory personnel and improve the work efficiency of batch sample processing. The lower half of the pipette (the part in contact with samples and reagents) adopts a detachable design, which can withstand high temperature and high pressure sterilization at 121℃ for 20 minutes, facilitating thorough purification and disinfection before and after use, eliminating cross-contamination between different involved evidence and different batches of experiments from the hardware level. It is especially suitable for the processing needs of forensic trace biological evidence and precious involved evidence, and maximally ensures the originality of the evidence and the authenticity of the test results.
The product is equipped with a complete calibration and maintenance system, providing free online calibration technical support and standardized calibration process guidance. Laboratory personnel can conveniently complete regular performance verification and range calibration of the equipment with the help of standard weights and purified water, ensuring that the pipette continuously meets the legal metrological requirements throughout the life cycle, ensuring the accuracy and stability of long-term use, and avoiding the invalidation of test results caused by the inaccuracy of metering instruments. At the same time, it provides 8-channel and 12-channel multi-channel pipette specifications, which are specially optimized for 96-well plate batch operation, equipped with a synchronous tip ejector, which can greatly improve the work efficiency of batch DNA extraction, PCR system preparation and sample processing for forensic DNA database construction. The liquid head supports 360-degree rotation, which can adapt to the operation needs of narrow spaces in biological safety cabinets and ultra-clean benches. Each channel is equipped with an independent piston, which is easy for daily maintenance and troubleshooting.
Compliant Application Scenarios
This product can only be operated in a standardized manner by professionally trained personnel in criminal technology laboratories and judicial authentication institutions with statutory inspection qualifications. The core compliant application scenarios include four categories: First, forensic DNA extraction and PCR reaction system construction. In DNA extraction processes such as magnetic bead method and column extraction method, it can accurately transfer lysate, binding solution, washing solution and eluent. In the preparation of STR typing and PCR amplification reaction system, it can accurately add template DNA, primers, probes, dNTPs and DNA polymerase, which is the core basis for obtaining effective DNA typing maps and completing individual identification. Second, quantitative analysis of toxic drugs and physical and chemical analysis, which is used to prepare standard solutions and internal standard solutions of toxic (drugs) with different concentrations, and accurately transfer the supernatant of biological samples such as blood and urine involved in the case, to ensure the accuracy of qualitative and quantitative analysis results of instruments such as gas chromatography and liquid chromatography-mass spectrometry. Third, serological and immunological testing, in enzyme-linked immunosorbent assay, blood group identification, antibody-antigen reaction and other experiments, it is used to accurately distribute samples, antibodies, enzyme substrates and other reagents to ensure the standardization of reaction conditions and repeatability of results. Fourth, trace evidence processing and laboratory standard system construction, which is used for the accurate transfer of extraction solvents for trace evidence, as well as the preparation of various standard curve solutions, buffer systems and quality control samples in daily laboratory work, supporting the stable operation of the standardized quality system of criminal technology laboratories.
Core Technical Parameters
The product range covers 0.1μl to 10000μl (10ml), which fully adapts to the needs of ultra-micro, micro and constant liquid processing in the laboratory. The factory calibration and regular calibration strictly follow the EN/ISO 8655 international metrology standard, and the pipetting accuracy is better than the standard requirements. The channel configuration includes single-channel adjustable pipettes, as well as 8-channel and 12-channel multi-channel pipettes adapted to 96-well plate operation. It is equipped with a high-definition digital window, the range setting is clear and intuitive, the lower half can withstand high temperature and high pressure disinfection at 121℃, and the overall adopts a lightweight design in line with ergonomics. The multi-channel pipette is equipped with a synchronous tip ejector, the liquid head supports 360-degree rotation, and each channel is equipped with an independent piston for daily maintenance and performance calibration.
Practical Application Cases
- The Criminal Technology Detachment of the Municipal Public Security Bureau accepted a series of burglary cases. Only trace contact exfoliated cell samples on door and window handles and beverage bottles were extracted from dozens of crime scenes. The DNA content in the samples was extremely low, which belonged to difficult trace biological samples, with extremely high requirements for pipetting accuracy in PCR reaction system preparation. The laboratory inspectors used this series of single-channel adjustable micro pipettes to accurately complete the pipetting and system preparation of reagents such as template DNA, primers and polymerases at the level of 1μl. The cross-contamination was eliminated through the high-temperature sterilizable lower half design throughout the process, and the pipetting accuracy fully complied with the ISO metrology standard. Finally, the complete STR typing map of the suspect was successfully obtained from 12 trace samples. After comparison with the national forensic DNA database, the suspect Zhang with previous criminal record was successfully locked, and more than 40 cross-regional theft cases in this series were solved. The high-precision pipetting ability and anti-cross contamination design of this product provided key basic support for the successful inspection of difficult trace samples.
- A major poisoning case occurred in a certain city. The victim died of poisoning after eating the poisoned food. The forensic doctor extracted the victim's gastric contents, blood, remaining food and other involved evidence, which needed to be qualitatively and quantitatively analyzed by liquid chromatography-mass spectrometry. During the inspection, it was necessary to prepare gradient concentration poison standard solutions and internal standard solutions, and the accuracy of pipetting was extremely high, which directly affected the judicial validity of the quantitative results. The inspectors used this series of pipettes to accurately complete the gradient dilution and pipetting of standard products with different concentrations, and completed the performance verification before pipetting in strict accordance with the calibration process to ensure the accuracy of standard curve preparation. Finally, the highly toxic pesticide components were successfully detected in the samples, and accurate quantitative analysis was completed, which provided scientific, accurate and traceable quantitative inspection results for the case characterization and judicial litigation, and helped the public security organ quickly arrest the poisoning suspect and complete the full chain investigation of the case.